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Beilstein J. Org. Chem. 2014, 10, 2348–2360, doi:10.3762/bjoc.10.245
Graphical Abstract
Figure 1: Structures explaining why large DNA (L-DNA) constructs cannot easily autonomously self-assemble fro...
Figure 2: Watson–Crick pairing rules follow two rules of complementarity: (a) size complementarity (large pur...
Figure 3: (top) Schematic showing the mix-anneal-extend-ligate-amplify process exploiting AEGIS pairing (to o...
Figure 4: The exact sequences and overlaps of the fragments designed by OligArch to allow the autonomous asse...
Figure 5: Agarose gel (1%, stained with ethidium bromide) showing the products (before PCR) arising from the ...
Figure 6: LB-agar plates spread, at various dilutions, with 25 µL cells transformed by plasmids containing th...
Figure 7: (left) Autonomous assembly of the 32A construct yielded the desired 1,121 base-pair amplicon in a s...
Figure 8: Fragments built only from standard nucleotides, without AEGIS, and designed by OligArch [21] to support...